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Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers
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STANDARD published on 1.1.2023
Designation standards: ASTM E3029-15(2023)
Publication date standards: 1.1.2023
SKU: NS-1099222
The number of pages: 5
Approximate weight : 15 g (0.03 lbs)
Country: American technical standard
Category: Technical standards ASTM
Keywords:
calibration, fluorescence, fluorometer, luminescence, qualification, reference materials, spectral correction, spectrometer, spectroscopy, validation,, ICS Number Code 17.180.01 (Optics and optical measurement in general)
Significance and Use | ||||||||
3.1?Calibration of the responsivity of the detection system for emission (EM) as a function of EM wavelength (?FIG. 1?Example of Relative Spectral Responsivity of Emission Detection System (Grating Monochromator-PMT Based), (see Test Method E578) for which a Correction Needs to be Applied to a Measured Instrument-Specific Emission Spectrum to Obtain its True Spectral Shape (Relative Intensities). 3.2?When using CCD or diode array detectors with a spectrometer for ?3.3?Instrument manufacturers often provide an automated procedure and calculation for a spectral correction function for emission, or they may supply a correction that was determined at the factory. This correction can often be applied during spectral collection or as a post-collection correction. The user should be advised to verify that the automated vendor procedure and calculation or supplied correction are performed and determined according to the guidelines given within this standard. |
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1. Scope | ||||||||
1.1?This practice (1.2?The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3?This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4?This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. |
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2. Referenced Documents | ||||||||
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