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ASTM E579-04(2023)
Standard Test Method for Limit of Detection of Fluorescence of Quinine Sulfate in Solution
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STANDARD published on 15.1.2023
The information about the standard:
Designation standards: ASTM E579-04(2023)
Publication date standards: 15.1.2023
SKU: NS-1100840
The number of pages: 3
Approximate weight : 9 g (0.02 lbs)
Country: American technical standard
Category: Technical standards ASTM
The category - similar standards:
Annotation of standard text ASTM E579-04(2023) :
Keywords:
fluorescence spectrometers, molecular luminescence, molecular spectroscopy,, ICS Number Code 71.080.30 (Organic nitrogen compounds)
Additional information
| Significance and Use | ||
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4.1?When determining the limiting detectable concentration of a fluorescent substance, it is usually necessary to increase the readout scale of a photoelectric instrument to a point where noise (that is, random fluctuations of the system) becomes apparent. This noise will be superimposed upon the signal from the sample. 4.2?In molecular fluorescence spectroscopy, the limit of detection for the sample will be determined by the limiting signal-to-noise ratio, Note 2:?Factors other than noise affecting the sample concentration corresponding to the limit of detection include: the spectral bandwidths of the excitation and emission monochromators, the intensity of the exciting light that can be concentrated on the sample, the fraction of the fluorescence collected by the detection system, the response time of the detection system, and the purity of the solvent. The size and arrangement of the sample container with respect to the light beams are also important, as they affect both the desired signal and the extraneous signal that only contributes noise. 1.1?This
test method employs the signal-to-noise ratio to determine the
sensitivity of a fluorescence measuring system in testing for the
limit of detection (LOD) of quinine sulfate dihydrate in solution.
The results obtained with quinine sulfate dihydrate in solution are
suitable for specifying instrument performance on samples having
excitation and fluorescence bands wider than 10 nm at or near room
temperature.
1.1.1?This test method is not intended to be used as (1.2?This test method has been applied to fluorescence-measuring systems utilizing non-laser, low-energy excitation sources. There is no assurance that extremely intense illumination will not cause photodecomposition1.3?A typical LOD for conventional fluorometers using this test method is 1 ng of quinine sulfate per mL. 1.4?The suggested shelf life of a 1 mg/mL stock solution of quinine sulfate dihydrate is three months, when stored in the dark in a stoppered glass bottle. 1.5?The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6?This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7?This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. |
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| 2. Referenced Documents | ||
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