ČSN EN ISO 15216-1 (560087)

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification

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STANDARD published on 1.10.2017


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The information about the standard:

Designation standards: ČSN EN ISO 15216-1
Classification mark: 560087
Catalog number: 502911
Publication date standards: 1.10.2017
SKU: NS-796821
The number of pages: 64
Approximate weight : 192 g (0.42 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

The category - similar standards:

Food microbiology

Annotation of standard text ČSN EN ISO 15216-1 (560087):

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices

These changes apply to this standard:

ČSN EN ISO 15216-1:2017/A1 (560087) Change

Mikrobiologie potravinového řetězce - Horizontální metoda pro stanovení viru hepatitidy A a noroviru pomocí RT-PCR v reálném čase - Část 1: Metoda pro kvantitativní stanovení (Norma k přímému použití jako ČSN).

Change published on 1.10.2021

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