ČSN EN ISO 23418 (560057)

Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of bacteria - General requirements and guidance

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STANDARD published on 1.12.2022


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The information about the standard:

Designation standards: ČSN EN ISO 23418
Classification mark: 560057
Catalog number: 515653
Publication date standards: 1.12.2022
SKU: NS-1091100
The number of pages: 60
Approximate weight : 180 g (0.40 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

The category - similar standards:

Food microbiology

Annotation of standard text ČSN EN ISO 23418 (560057):

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:

  • a) handling of bacterial cultures;
  • b) axenic genomic DNA isolation;
  • c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
  • d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
  • e) metadata capture and sequence repository deposition;
  • f) validation of the end-to-end WGS workflow (fit for purpose for intended application).

This document is applicable to bacteria isolated from:

  • - products intended for human consumption;
  • - products intended for animal feed;
  • - environmental samples from food and feed handling and production areas;
  • - samples from the primary production stage

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